医药学论文：龙寿丹对急性脑梗塞患者血清肿瘤坏死因子及循环内皮细胞含量的影响

内容提要　目的：研究龙寿丹对急性脑梗塞(ACI)患者血清肿瘤坏死因子(TNF)及循环内皮细胞(CEC)含量的影响，进一步探讨龙寿丹治疗脑梗塞有效的作用机制，为龙寿丹治疗脑梗塞的临床应用提供理论依据。方法：采用放射免疫分析法检测40例ACI患者服用龙寿丹前后血清TNF-α和CEC的含量，并与20例未服用龙寿丹的ACI患者及20名健康人作对照。结果：(1)ACI患者血清TNF-α和CEC含量明显高于健康组，且增高的程度与梗塞灶大小密切相关，梗塞灶越大，其含量越高。(2)ACI患者血清TNF-α与CEC呈正相关(r＝0.68，P＜0.01)。(3)4周后两组ACI患者血清TNF-α及CEC含量均降低，但治疗组降低更明显，与对照组比较具有显著性差异(P＜0.01)。结论：龙寿丹降低了ACI患者血清中增高的TNF-α及CEC，减轻了脑血管内皮细胞的损害，对脑血管内皮细胞及缺血神经元具有保护作用。

　　Objective: To investigate the effect of Longshoudan (LSD) on the serum tumor necrosis factor (TNF) and circulating endothelial cell (CEC) levels in acute cerebral infarction (ACI) patients, and the mechanism of LSD in treating ACI.Methods: The levels of serum TNF-α and CEC in 40 ACI patients before and after LSD oral administration were measured by radioimmunoassay, and compared with 20 routinely treated ACI patients, and 20 healthy subjects as control group.Results: (1)The levels of serum TNF-α and CEC in ACI patients were significantly higher than those in the control group, there was significant correlation between the increas ed extent and infarction size, the larger the size, the higher the levels of serum TNF-α and CEC; (2)In ACI patients, the serum TNF-α level was positively correlated with CEC level (r=0.68,P＜0.01); (3)The serum TNF-α and CEC level of all ACI patients after 4 weeks were reduced, and LSD treated group was significantly lower than that of routinely treated group (P＜0.01).Conclusion: LSD could protect cerebrovascular endothelial cells and ischemic neurons through lowering the increased levels of serum TNF-α and CEC in ACI patients, and alleviate the damage of cerebrovascular endothelial cells